Dr. Danielli specializes in Electro-optics and Bio-photonics. After completing his Bachelor’s and Master’s degrees in the field of optical communication at Tel Aviv University, he worked in industry for eight years and then return to the Academy. During his studies for P.hD, he developed a new technology to rapidly detect fluorescent-labeled probes at very low concentrations in homogenous solutions and continued developing it during his post-doctoral studies in Washington University, St. Louis.
In 2014, he has returned to Israel and joined the faculty of Engineering at Bar Ilan University.
During his stay in the US, he founded a company that commercializes this technology, and established connections with key figures in the fields of medical laboratory and diagnostics, and especially among troponin and heart attack specialists.
. In these assays, usually a target molecule is captured using a probe conjugated to a capture surface and then detected using a second fluorescently labeled probe. One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical performence of the assay. In this research, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. We analyzed their autofluorescence properties and the stability of the photobleaching over a period of two months. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200™ system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in detection limit and signal to noise ratio over results achievable with non-bleached beads.
Congratulations to our Ph.D student, Michael Margulis, for the publication of his paper in the Journal of Biophotonics. This is his second paper as a first author which has been published in the framework of his Ph.D studies.
The paper, entitled 'Detecting nucleic acid fragments in serum using a magnetically modulated sandwich assay
', can be found in the Publications section, and at DOI: 10.1002/jbio.201900104.
In his effort to promote a collaboration with Japanese, Dr. Danielli visited researchers and clinicians from the Emergency Department of the RyuKyu University. During the visit, Dr. Danielli presented the research that is conducted in the Biosensing and Imaging Laboratory at Bar Ilan University, including "Sensitive and specific detection of flaviviruses, such as the Zika, dengue, and West-Nile viruses" and "Highly sensitive detection of protein protein interactions". The visit was very fruitful and will potentially lead to future collaborations between the institutes.
More newsWe are always happy to hear from postdocs and students at all levels (BSc., MSc. and PhD.) with various backgrounds (mathematics, engineering, physics, biology) who are interested by the kind of research we are doing. send an email to Amos.danielli2@gmail.com
